Clinical Evaluation of GuruNanda® Natural Oil Pulling Oral Rinse versus CPC-Based Mouthwash
A comparative clinical evaluation of oral health and gingivitis management
Objective
The objective of this randomized, single-blind, placebo-controlled clinical study was to evaluate and compare the efficacy and safety of GuruNanda® Natural Oil Pulling Oral Rinse with a CPC-based mouthwash and placebo in adults with mild-to-moderate gingivitis. The study assessed improvements in gingival health, plaque accumulation, oral microbial balance, breath freshness, and overall oral hygiene over an eight-week period.
The GuruNanda® formulation is a proprietary blend of coconut, sesame, sunflower, and peppermint oils enriched with Ashwagandha extract, designed to support oral health through natural antimicrobial, anti-inflammatory, and antioxidant mechanisms.
Process
A total of 66 adults aged 18–60 years diagnosed with mild-to-moderate gingivitis were enrolled following ethics committee approval and CTRI registration. Participants were randomized into three equal groups (n = 22 per group):
- Group A (Test): GuruNanda® Natural Oil Pulling Oral Rinse, 10 mL once daily
- Group B (Comparator): CPC-Based Mouthwash, 15 mL twice daily
- Group C (Control): Placebo rinse, 10 mL once daily
Clinical evaluations were performed at baseline, Week 4, and Week 8. Outcome measures included the Modified Gingival Index (MGI), Plaque Index (PI), microbial counts (Streptococcus mutans, Gram-positive and Gram-negative bacteria), tooth shade (VITA scale), Clinical Oral Dryness Score (CODS), and Organoleptic Scoring Scale (OSS).
Safety assessments included oral examinations, vital signs, hematology, biochemistry, and adverse event monitoring. Statistical analysis was conducted using paired and independent-sample t-tests, with statistical significance set at p < 0.05.
Results
Table 1. Change in Gingival and Plaque Indices Across Study Visits
| Parameter | Group | Baseline Mean ± SD |
Week 4 Mean ± SD |
Week 8 Mean ± SD |
% Reduction | Significance vs Placebo |
|---|---|---|---|---|---|---|
| Modified Gingival Index | GuruNanda® | 2.14 ± 0.51 | 1.70 ± 0.42 | 1.49 ± 0.36 | 30.3% | p < 0.001 |
| Modified Gingival Index | CPC-Based | 2.12 ± 0.47 | 1.80 ± 0.38 | 1.65 ± 0.33 | 22.2% | p < 0.001 |
| Modified Gingival Index | Placebo | 2.11 ± 0.52 | 2.04 ± 0.51 | 2.14 ± 0.52 | −0.1% | — |
| Plaque Index | GuruNanda® | 2.56 ± 0.53 | 1.99 ± 0.37 | 1.73 ± 0.34 | 32.4% | p < 0.001 |
| Plaque Index | CPC-Based | 2.53 ± 0.46 | 2.19 ± 0.35 | 2.00 ± 0.32 | 20.9% | p < 0.001 |
| Plaque Index | Placebo | 2.53 ± 0.52 | 2.47 ± 0.50 | 2.50 ± 0.51 | 0.0% | — |
Table 2. Microbial and Whitening Outcomes After 8 Weeks
| Parameter | GuruNanda® | CPC-Based | Placebo |
|---|---|---|---|
| Reduction in S. mutans | Significant (p < 0.001) | Significant (p < 0.001) | None |
| Gram-Positive Flora | Maintained (balanced) | Decreased | No change |
| Gram-Negative Flora | Reduced (p < 0.001) | Reduced (p < 0.001) | No change |
| Teeth Whiteness (VITA) | Improved in 28% subjects | No change | No change |
| CODS / OSS | No dryness; pleasant mouthfeel | Mild dryness reported | No change |
Figure 1. Comparative changes in Modified Gingival Index and Plaque Index across baseline, Week 4, and Week 8 visits, demonstrating significant reductions with GuruNanda® Natural Oil Pulling Oral Rinse.
Figure 2. Changes in Streptococcus mutans and Gram-negative bacterial counts across study groups, showing effective microbial reduction with superior balance in the GuruNanda® group.Observation
GuruNanda® Natural Oil Pulling Oral Rinse demonstrated significant clinical improvements in gingival inflammation and plaque accumulation comparable to the CPC-based mouthwash, while offering superior tolerability and user experience. Microbiological findings indicated selective suppression of harmful bacteria with preservation of beneficial oral flora.
Participants consistently reported smoother mouthfeel and improved freshness compared with CPC and placebo groups, with no adverse reactions observed.
Conclusion
This clinical study confirms that GuruNanda® Natural Oil Pulling Oral Rinse is a safe, effective, and well-tolerated natural alternative to CPC-based chemical mouthwashes for managing gingivitis and maintaining oral health. Its balanced antimicrobial profile, absence of adverse effects, and enhanced user satisfaction support its suitability for long-term daily oral care.